Induced Ammonia Stress on Development of Albino Rat through Study of Certain Biochemical Components

Fertilizers have a significant role in the everyday life of modern cultivation. The metabolic changes and potentialities of ammonia in high concentration are reported in all groups of animals as ammonia toxicity and stress. The ammonia based chemicals might affect the reproductive organs of fetus when the mammals get exposed during pregnancy. The present aim of the research is to study some of the important physiological and biochemical events occurring under sub lethal concentrations of ammonia as a function of a chronic stress in Wistar strain albino rat. Lethal doses of ammonium sulphate were determined by probit method of Finney (1971). Female Rats were exposed intraperitonially to1/5 of the LD50 dose i.e., 62 mg/kg body weight till delivery. Selected tissues namely liver, brain and kidney were taken for biochemical study in control and both ammonia exposed females and newly born litter from the exposed female .Glutamine, glutamate dehydrogenase enzyme levels(GDH) and glutamine synthetase enzyme levels were estimated in the above tissues. There was increment in all the parameters selected in both female and litters tissue levels.